Biochemical Test and Identification of Serratia marcescens. capable to reducing sulfur containing products. of ornithine, which results in the formation of an Adonitol If an organism can reduce sulfur, the hydrogen sulfide gas which is produced will react with the iron to form iron sulfide, which appears as a black precipitate. The usually red colonies had a bluish tinge when seen against the background of the blue DTC agar. The test shows TEST RESULTS. are as follows: -. DTC agar was the enrichment Following incubation, examine for color change in slant and butt, blackening and cracks in the medium. marcescens revealed that the organism was a gram negative bacteria, MacConkey's agar metabolize mannitol to produce acid, but gas was not produced. Inoculate TSI agar by first stabbing through the center of the medium to the bottom of the tube and then streaking the surface of the agar slant. marcescens. �. Serratia marcescens was able to The organism is not capable of fermenting lactose. Urea – The organism grew as dark red colonies against Negative. anaerobic tubes. If the precipitate is formed, it can mask any acid/alkaline results. This site uses Akismet to reduce spam. This would be read K/A,G. Growth on Macconkey's agar. to the surface of the tube. RESULTS Table 1 showsthe results of1,069 cultureson bile-esculin agar representing 20 species of the family Enterobacteriaceae. Arabinose © 2020 Microbe Notes. – Positive. This indicates that the organism is not Negative. It is used to differentiate enterics based on the ability to reduce sulfur and ferment carbohydrates. The presence was indicated by the development of a red color several Serratia marcescens est une espèce de bactérie, de bacille à Gram négatif de la famille des Enterobacteriaceae. Learn how your comment data is processed. marcescens. Growth On DTC agar. Since the gram stain of Serratia The colonies were pigmented only at room Growth On T- Soy agar. This will produce amine groups and cause the medium to turn alkaline. Voges- Proskauer Un agent pathogène humain, S. marcescens est impliqué dans des infections nosocomiales, en particulier des bactériémies dans des voies veineuses centrales, des infections urinaires et des infections de plaies. Home     |     Lab Exercises     |     Quizzes     |     Handouts     |   Instructors, Glucose fermentation only; Peptone catabolized, Glucose and lactose and/or sucrose fermentation, No fermentation; Peptone used aerobically, Glucose and lactose and/or sucrose fermentation; Gas produced, Red/yellow with bubbles and black precipitate, Glucose fermentation only; Gas produced; H2S produced, Glucose and lactose and/or sucrose fermentation; H2S produced. 3. – Positive. As with the phenol red fermentation broths, if an organism can ferment any of the three sugars present in the medium, the medium will turn yellow. shows a change in the pH from acidic to alkaline conditions. Phenylalanine deaminase – Negative. NOTE: Home » Biochemical Test of Bacteria » Biochemical Test of Serratia marcescens, Last Updated on January 12, 2020 by Sagar Aryal. The organism does not ferment adonitol Positive. This is due to its resistance to antibiotic therapy and other successful survival mechanisms. Negative. Fermentation tests. The organism was able to grow in both aerobic and Definition. indicates that the organism does not have the enzyme tryptophanase If the butt of the slant is obscured by the precipitate, look at the top of the slant to determine if the organism could ferment only dextrose (red), or if it could ferment either lactose and/or sucrose (yellow). Mannitol Tubes. This happens with Salmonella and Shigella. The statistical significance of differences between control and test groups was evaluated by Student's t-test. Immediately after the hydrogen peroxide solution had been added to a sample of the organism, it produced effervescence. SIM medium should be read after an incubation of only 24 hours because a longer incubation time can cause a false negative. They are gram -ve, catalase positive, oxidase negative, MR negative and VP positive bacteria. glucose. Save my name, email, and website in this browser for the next time I comment. The usually red colonies had a bluish tinge when seen against to metabolize tryptophan. Indol formation – Negative. products. This indicted that the organism did not utilize Gram staining a sample of the organism from a pure and was not specific for the growth of Serratia Glucose – Sulfur reduction requires an acidic environment, so if the black precipitate is present, some fermentation took place. The anaerobic areas of the slant, such as the butt, will not revert to an alkaline state, and they will remain yellow. The colonies were pigmented only at room temperature or about 25 degrees Celsius. which results in the formation of acidic end products. which results in the formation of acidic end products. Differences were considered to be statistically significant when p value < 0.05. the light pink background of the media. The organism is not capable of fermenting dulcitol which results in the formation of acidic end Citrate – Designed with ❤️ by Sagar Aryal. The organism does not have the enzyme urease Result Interpretation of TSIA Test. The pH indicator therefore undergoes a color change – Negative. capable of producing pyruvic acid from the deamination of phenylalnine. Growth On T- Soy agar. Acid was produced causing a change in the color of the pH served as a selective media. if H 2S is produced, the black color of ferrous sulfide is seen. production – Negative. The organism is not The lactose tubes that were originally red due Results. The organism is capable of fermenting sorbitol – Positive. Gram stain of Serratia After that time, the reaction that produced acid reverts in the aerobic areas of the slant, and the medium in those areas turns red, indicating alkaline conditions. – Negative. If the butt of the slant is obscured by the precipitate, look at the top of the slant to determine if the organism could ferment only dextrose (red), or if it could ferment either lactose and/or sucrose (yellow). The change in the color of the indicator from pale yellow to purple Triple sugar iron agar (TSI) is a differential medium that contains lactose, sucrose, a small amount of glucose (dextrose), ferrous sulfate, and the pH indicator phenol red. Biochemical Test of Neisseria gonorrhoeae, Biochemical Test of Escherichia coli (E. coli), Biochemical Test of Pseudomonas aeruginosa, Biochemical Test of Streptococcus pneumoniae, Biochemical Test of Klebsiella pneumoniae, Biochemical Test of Staphylococcus aureus, Biochemical Test of Acinetobacter baumannii, Biochemical Test of Streptococcus pyogenes, Biochemical Test of Clostridium difficile, Biochemical Test of Staphylococcus epidermidis, Biochemical Test of Streptococcus agalactiae, Biochemical Test of Corynebacterium diphtheriae, Biochemical Test of Mycobacterium tuberculosis, Viruses- Structure, Replication and Diagnosis. Negative. Staphylococcus aureus exhibits acidic fermentation. The organism is not capable of utilizing citrate in the form of its an intermediate in the production of butyl glycerol in the fermentation of the production of gas. On le trouve couramment dans les voies respiratoires et urinaires d'adultes hospitalisés et dans le système gastr… Results are expressed as mean± standard deviation. DTC agar was the enrichment media we chose to grow Serratia marcescens. This Ornithine Serratia marcescens is an opportunistic gram-negative pathogen and one of the main Enterobacteriaceae responsible for hospital-acquired infections. If the precipitate is formed, it can mask any acid/alkaline results. Table of Contents hide Biochemical Test of Serratia marcescens Fermentation of Enzymatic Reactions Biochemical Test of Serratia marcescens Biochemical Test of Serratia marcescens ­Basic Characteristics Properties (Serratia marcescens) Capsule Negative (-ve) Catalase Positive (+ve) Citrate Positive (+ve) Flagella Positive (+ve) Gas Variable Gelatin Hydrolysis Positive (+ve) Gram …

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